scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation.

Features 1,300 highly relevant proteins are profiled in a single assay Phosphorylation status combined with protein expression levels Tyrosine, serine and threonine phosphorylation Analysis of individual phosphorylation types available "Sample-to-result" service Comprehenisive data analysis and presentation Individualised study report

Advantages High-content screening of proteins and phosphorylation is time and ressource efficient Direct comparability through protein expression and phosphorylation level analysis in a single assay Complete analysis from minimal sample volume Coverage of important Signalling pathways Transcription factors Receptor molecules Kinases Individual data analysis options available Protein interaction network analysis Biological and pathway classification  Lists with differential proteins and phosphorylation levels Variety of sample formats possible without the potential bias of a sample fractionation or a sample depletion Results presented in a publication-ready format

Applications T cell activation Tissue treatment response Cell cycle regulation Oxidative stress response Cell adhesion and motility Phosphorylation profiling of various signalling pathways: FAK phosphorylation PI3K-AKT pathway p53 pathway regulation Alzheimer pathway regulation

Case study   Target proteins   Contact us

Case study

Eight treated cell line samples were compared with 8 control samples. Several proteins were identified as differentially expressed (x-axis) and/or phosphorylated (y-axis). While the left plot provides an overview on expression / phosphorylation differences for all tested proteins in the two sample groups, individual levels are depicted in the plots on the right. The sample status is indicated as C = control or T = treated.

Reference customers

Prof. Dr. Mathias Heikenwälder, German Cancer Research Center, Heidelberg, Germany

Dr. Caroline Pabst. University Hospital Halle, Germany

More information

Download our scioPhospho or scioDiscover service brochure.

Information on protein and pathway content / coverage.

Get in touch to answer any remaining questions.

Ask for a quotation to get your study started.

Which sample types can be analysed?

With the scioPhospho service a variety of samples can be analysed:

• fresh frozen tissue samples
• cellular content
• cerebrospinal fluid
• plasma / serum
• additional sample types on request

Sample-to-result service

Within our analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

• definition of an appropriate study design
• sample preparation
• protein extraction
• protein concentration measurements
• protein quality control
• sample labelling
• sample purification
• incubation of the samples on antibody microarrays
• microarray scanning
• raw data acquisition
• data normalisation
• data analysis including cluster analysis
• statistical testing for differentially abundant proteins
• statistical testing for differentially phosphorylated proteins
• comprehensive study report

You identified a compound with the desired effect ?

You are looking for its target(s) ?

Which additional pathways are affected ?

Which proteins are altered in a diseased tissue ?

Get the whole picture of protein expression in multiple pathways !

Profound knowledge about protein expression levels is of utmost importance for the discovery and evaluation of new drug targets. Our Scio-Discover Service is fully immuno-based and covers proteins involved in major disease and signalling pathways as well as secreted proteins. For the highly-parallel protein analysis only minimal amounts of sample are required. No sample fractionation or depletion is needed. Our platform is able to process most biological samples without the need for laborious sample preparation, saving time and resources.

Applications:

• Drug Target Identification and verification studies
• Mechanistic studies for various indications
• Correlate Next-Gen-Sequencing Data to protein expression levels
• Profile cytokine and inflammatory status of your samples

Key advantages:

• Low sample consumption 
• Investigation of human, murine and rat samples*
• Fast turnaround time, as low as two weeks**
• No need for laborious depletion or fractionation of your samples
• High sensitivity (as sensitive as an ELISA or better)
• High reproducibility (CV < 10%)
• Various sample types can be investigated
  • Serum / Plasma
  • Tissue
  • Cell culture
  • Cerebrospinal fluid (CSF)

Service Features:

• Study Layout
• Protein Extraction and Purification
• Protein Quantification
• Protein labelling with high-performance fluorescent dyes
• Automated array incubation and handling 
• Array scanning and data acquisition
• Data analysis according to your requirements
• Individual study report

Overview
Scio-Discover     Array
Content     Biomarker
Discovery     Contact
us

_{* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].}

_{** depending on study design and sample numbers}

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised range of protein profiling services available. These help you to speed up your research and generate important data at lower cost through a highly-parallel protein expression level analysis using our Scio-Discover Antibody Microarray Platform. Furthermore, you may link to and verify your Genomics and Transcriptomics data with protein expression levels.

Applications:

• Pre-clinical Toxicology Studies
  • rodents such as mice* and rats*
  • cell culture models
  • tissue culture models
• Drug target identification and verification
• Mechanistic studies for various indications
• Profile cytokine and inflammatory status
• Protein Biomarker identification and verification

Key advantages:

• High multiplexity (1,300 proteins in parallel)
• Low sample consumption
• Investigation of human and murine samples*
• Fast turnaround time, as low as two weeks**
• No need for laborious depletion or fractionation of your samples
• High sensitivity (as sensitive as an ELISA or better)
• High reproducibility (CV < 10%)
• Various sample types can be investigated
  • Serum / Plasma
  • Tissue
  • Cell culture
  • Cerebrospinal fluid (CSF)

Overview
Scio-Discover     Pathway
Coverage     Biomarker
Discovery     Contact
us

* Antibody array is designed to target human proteins. Due to a high sequence homology of vast majority of proteins it was often successfully applied to study murine, rat and ovine models as well  [Thorenz A. et al. (2018), Peiro J. et al. (2018), Reichman H. et al. (2019), Raquel F. et al. (2020)].

.** depending on study design and sample numbers

Characterise a certain cell type?

Cell composition in a certain tissue sample?

In-depth profiling of CD-marker expression?

Increase your throughput at high sensitivity levels?

You aim at high reproducibility?

Our scioCD profiling service covers 260 different proteins :

• up to 141 unique CD-marker molecules
• up to 119 Cytokines/Chemokines
• other relevant proteins such as various HLAs and p53

All these proteins can be analysed in parallel within a single experiment from minute sample amounts. Thereby, scioCD antibody array analysis service is ideally suited to investigate cell composition of tissues or the differentiation status of cultured cells in a highly parallel, fast and robust fashion. In addition, protein distribution in blood samples (plasma / serum) can be analysed efficiently, providing insights in the blood cell composition.

scioCD is a complete sample-to-result service for a variety of different sample types. The respective protocols were developed over a period of 15 years and are optimised towards highest possible sensitivity combined with high reproducibility (coefficients of variation below 10%).

Target
proteins       Analysis
Service       Sample
Types       Product
brochure       Contact
us

Target list (version 1) for the scioCD protein profiling service.

Extended version 2 to follow soon ! Contact us for the updated target overview via eMail This email address is being protected from spambots. You need JavaScript enabled to view it.

Which sample types can be analysed ?

Within our scioCD analysis service the following samples can be analysed:

• plasma / serum
• fresh frozen tissue samples
• cellular content
• cell culture supernatant
• cerebrospinal fluid
• additional sample types on request

Sample-to-result service

Within the analysis service, we will not only carry out the microarray experiments but also support you with a suggestion for an appropriate microarray study design as well as with the sample selection process in order to address your scientific question in the optimal way. Within 3-4 weeks after receipt of your samples you will receive a customised study report including a statistical analysis.

Our analysis service includes:

• definition of an appropriate study design
• sample preparation
• protein extraction
• protein concentration measurements
• protein quality control
• sample labelling
• sample purification
• incubation of the samples on antibody microarrays
• microarray scanning
• raw data acquisition
• data normalisation
• data analysis including cluster analysis
• statistical testing for differentially abundant proteins
• comprehensive study report