Target proteins

Our scioDiscover and scioPhospho services are based on complex Antibody Microarrays, also called analytical Protein Arrays or Protein Capture Arrays. Within a single experiment 1030 proteins can be profiled in parallel requiring only minimal amounts of sample. The targets proteins were carefully selected over a period of more than 15 years to cover a broad set of different protein classes as well as pathways important in biomedical studies.

Thereby, the protein profiling covers key pathways in various diseases such as cancer, neurological disorders as well as organ failure. Given the wide spectrum of applications and indications the service is ideal for high-content protein expression level analysis from various biological samples. The broad coverage of many important pathways, extracellular and membrane proteins as well as transcription factors, renders scioDiscover a versatile and efficient tool for discovery projects.

Please find below the distribution of the proteins targeted by scioDiscover according their biological processes (Fig.1), their protein classes (Fig.2), their molecular function (Fig.3) as well as their cellular compartment (Fig.4). In addition, pathways covered by scioDiscover are listed in Table 1.

For the pathways TNF signaling, Cell cycle regulation and pathways important in Cancer, Tuberculosis and Huntington's Disease covered targets are depicted in green.

Please contact us for more information regarding targets and pathways covered or to discuss how we can support your study with a highly-parallel protein analysis.

 

Case study - Bladder Cancer

Case study - Acute Kidney Injury

 

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Classification of target proteins

Biological processes

Protein classes

biological processes covered by protein array analysis

protein classes covered by protein array analysis

Fig. 1: Distribution of target proteins within different biological processes. The majority of the antibodies on the microarray detect proteins involved in cellular or metabolic processes or are involved biological regulation, in a developmental or immune system process or as a response to a stimulus.

Fig. 2: Distribution of target proteins within different protein classes. With scioDiscover 122 signaling molecules, 113 receptors, 109 nucleic acid binding proteins and 71 transcription factors can be analysed in parallel.

Molecular functions

Cellular compartmenrt

molecular functions covered by protein array analysis

 

cellular compartments covered by protein array analysis

 

Fig. 3: Distribution of target proteins within different molecular functions. The majority of proteins on the protein microarrays measures proteins involved in binding or with a catalytic or receptor activity.

Fig. 4: Target proteins classified according their Cellular Compartment.

 
 

Coverage of pathways

Selected Pathways no. targets
Inflammation mediated by chemokine and cytokine signaling (P00031) 40
Apoptosis signaling (P00006) 38
Gonadotropin releasing hormone receptor (P06664) 35
CCKR signaling map (P06959) 35
Integrin signalling (P00034) 29
Angiogenesis (P00005) 28
Interleukin signaling (P00036) 27
Huntington disease (P00029) 26
p53 pathway (P00059) 22
T cell activation (P00053) 22
B cell activation (P00010) 21
Ras Pathway (P04393) 18
Wnt signaling (P00057) 17
TGF-beta signaling (P00052) 17
EGF receptor signaling (P00018) 17
Alzheimer disease-presenilin (P00004) 15
FAS signaling (P00020) 15
FGF signaling (P00021) 14
VEGF signaling (P00056) 13
Toll receptor signaling (P00054) 13
Parkinson disease (P00049) 12
PDGF signaling (P00047) 12
Insulin / IGF pathway-mitogen activated protein kinase kinase / MAP kinase cascade (P00032) 12
Alzheimer disease - amyloid secretase  (P00003) 11
p53 pathway feedback loops 2 (P04398) 10
Endothelin signaling (P00019) 10
Blood coagulation (P00011) 10
Plasminogen activating cascade (P00050) 9
Cytoskeletal regulation by Rho GTPase (P00016) 9
Interferon-gamma signaling (P00035) 8
Insulin/IGF pathway-protein kinase B signaling cascade (P00033) 8
Ubiquitin proteasome (P00060) 7
Transcription regulation by bZIP transcription factor (P00055) 7
PI3 kinase (P00048) 7
Oxidative stress response (P00046) 7
Cadherin signaling (P00012) 7
 

 

Highly parallel protein analysis of TNF Signaling

Fig. 5: Proteins in the TNF signalling pathway covered by our protein array are shown in green.

 

 Parallel Analysis of Cell Cycle Proteins by complex antibody arrays

Fig. 6: Cell Cycle Proteins covered by antibody array are depicted in green.

 

 Cancer protein profiling

Fig 7: scioDiscover has a broad coverage of diverse pathways involved in cancer. Proteins in green can be profiled with scioDiscover antibody arrays.

 

 Protein analysis in Huntington's disease

Fig. 8: scioDiscover has a good coverage of proteins involved in various diseases such as Huntington's disease. Proteins analysed in parallel by scioDiscover protein arrays are shown in green

 

Proteins involved in Tuberculosis

Fig. 9: Pathways involved in Tuberculosis are shown. Proteins covered by the scioDiscover service are shown in green.

 

Our service portfolio

scioPhospho: protein profiling and phosphorylation status

scioPhospho: protein profiling and phosphorylation status

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation. Features 1,300 highly relevant proteins are profiled in a single assay Phosphorylation status combined with protein expression levels…
Read more
scioDiscover - Protein Profiling on expression level

scioDiscover - Protein Profiling on expression level

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…
scioCD - Cell surface marker and Cytokine profiling

scioCD - Cell surface marker and Cytokine profiling

  Characterise a certain cell type?   Cell composition in a certain tissue sample?   In-depth profiling of CD-marker expression?   Increase your throughput at high sensitivity levels? You aim…
scioUbi

scioUbi

Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse up to 1,000 proteins in a single assay.   Features Screen…
scioCyto : Cytokine profiling

scioCyto : Cytokine profiling

scioCyto : Cytokine profiling scioCyto is a high-content analysis service for multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or…

COVID-19 related activities and services

covid 19 portfolio web

News

New article | Cycle-dependent biomarkers in endometriosis

| May 2022 | In a study by Maja Pušić et al. Cycle-dependent plasma protein biomarkers for peritoneal endometriosis were identified by our scioDiscover platform.

Pušić, M. et al. Antibody Arrays Identified Cycle-Dependent Plasma Biomarker Candidates of Peritoneal Endometriosis. J. Pers. Med. 202212, 852.
New article | Co-Expression of microRNAs and Proteins in Alzheimer’s Disease

| Jan 2022 | In a study by Valentina di Pietro at Birmingham University our scioDoiscover platform was used to analyse the co-expression of proteins and microRNAs in the brains of Alzheimer's disease patients.

Watson, C. N. et al. Co-Expression Analysis of microRNAs and Proteins in Brain of Alzheimer’s Disease Patients. Cells 11, 163 (2022).
New article | ETV6 levels predict outcome in DLBCL

| Jan 2022 | Erich Piovan and collegues at University of Padua collaborated with Sciomics to study specific biomarkers in FFPE tissue samples of DLBCL patients by custom antibody microarrays.

Marino, D. et al. High ETV6 Levels Support Aggressive B Lymphoma Cell Survival and Predict Poor Outcome in Diffuse Large B-Cell Lymphoma Patients. Cancers 14, (2022).
New article | Biomarker identification and verification in endometriosis

| 22th Oct 2021 | Scientists at University Medical Centre Ljubljana used our scioDiscover platform for a biomarker identification in endometriosis:

"Discovery proteomics using antibody microarrays that covered 1360 proteins identified 16 proteins with different levels in cases versus the control patients. The validation using an ELISA approach confirmed significant differences in the levels of cartilage oligomeric matrix protein (COMP) and transforming growth factor-β-induced protein ig-h3 (TGFBI) and nonsignificant differences in angiotensinogen (AGT). "

 Janša, V. et al. (2021) ‘Proteomic analysis of peritoneal fluid identified COMP and TGFBI as new candidate biomarkers for endometriosis’, Scientific Reports, 11:20870. doi: 10.1038/s41598-021-00299-2. 

biomarkers in endometriosis

 

New article | Parkinson's Disease analysed via brain organoids

| Oct 2021 | In a study led by University of Luxembourg and OrganoTherapeutics SARL-S we used our scioPhospho assay to analyse the reaction of a brain organoid model for Parkinson's disease to certain treatments.

Jarazo, J. et al. (2021) ‘Parkinson's Disease Phenotypes in Patient Neuronal Cultures and Brain Organoids Improved by 2-Hydroxy...’, Movement Disorders, in press: doi: 10.1002/mds.28810 (Please get in touch for fulltext).

 

Testimonials

Dr. Joachim Lupberger 

Institute for Viral and Liver Disease, Inserm U1110, University of Strasbourg, LabEx HepSYS, Strasbourg, France

"Our lab is focusing on the identification of minimal-invasive biomarkers for liver disease progression. A challenge of our humanized animal models for liver disease is the low volume of blood samples available, which did not pose any problem for the analysis. I was pleased by the prompt, friendly, and uncomplicated contact and was impressed by the results and the thorough report we received. The overall quality was excellent, which made us to continue using the service of Sciomics for follow-up projects analyzing patient material."

Product: scioPhospho

 

 Prof. Dr. Andreas Weigert 

Goethe-University Frankfurt, Faculty of Medicine, Institute of Biochemistry I, Frankfurt, Germany

"We were under considerable time pressure to gain comprehensive information about cytokine/chemokine levels in a mouse model of self-resolving inflammation. To add to our misery, we had only a small number of biological replicates to run the analyses. Thankfully, the team at Sciomics were able to come to our rescue by providing a very prompt analysis, without neglecting to properly communicate the procedure, potential caveats and other details. The whole process was a very satisfying experience, rather like working with committed colleagues than with a company, and we got great data out of the project. I do happily recommend working with Sciomics for targeted proteomics analyses."

Product: scioCD

 

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