scioPhospho: protein profiling and phosphorylation status

Our scioPhospho analysis combines protein expression profiling using scioDiscover with analysis of the corresponding protein's phosphorylation status. More than 1,400 proteins are analyzed in parallel, covering key pathways in diseases such as cancer, neurological disorders and organ failure.  This combination provides a comprehensive overview of signaling events and pathway activity regulation in your sample. scioPhosho enables targeted, rapid, and cost-effective research.

Key Features

more than 1,400 highly relevant proteins are profiled in a single assay Combines analysis of phosphorylation status with protein expression level analysis Analysis of tyrosine, serine, and threonine phosphorylation Analysis of individual phosphorylation types is available 'Sample-to-result' service Comprehensive data analysis and reporting Customized study report

Advantages High-content screening of proteins and phosphorylation is time and resource efficient Direct comparability by analyzing the level of protein expression and the level of phosphorylation in a single assay Complete analysis from a minimal amount of sample Coverage of important Signaling pathways Transcription factors Receptor molecules Kinases Custom data analysis options available Protein interaction network analysis Biological and pathway classification  Lists of differentially expressed proteins and levels of phosphorylation Variety of sample formats possible without the potential bias of sample fractionation or sample depletion Results presented in a publication-ready format

Applications T-cell activation Tissue treatment response Cell cycle regulation Oxidative stress response Cell adhesion and motility Phosphorylation profiling of various signaling pathways FAK phosphorylation PI3K-AKT pathway p53 pathway regulation Alzheimer pathway regulation

Which Sample Types can be Analyzed?

A wide range of samples can be analyzed with scioPhospho

• Fresh-frozen tissue samples
• Cellular samples
• Cerebrospinal fluid (CSF)
• Plasma or serum
• Other sample types available upon request

A minimal amount of sample is required*, for example

• Plasma sample: 5µl
• Tissue: 1mg (fresh-frozen)
• Cells: 100,000
• CSF: 5µl

 Our Sample-to-Result Service

As part of our analysis service, we will not only perform the microarray experiments but will also support you with an appropriate microarray study design and sample selection to best address your scientific questions. After we receive your samples, you will receive a customized study report including statistical analysis within 3-4 weeks.

Our analysis service includes:

• Definining a suitable study design
• Sample preparation: Protein extraction, concentration measurements, quality control, and sample purification
• Microarray analysis
• Raw data acquisition and data normalisation
• Data analysis including cluster analysis
• Statistical testing for differentially abundant proteins
• Statistical testing for differentially phosphorylated proteins
• Comprehensive study report

Contact us 

Case Study

Eight treated cell line samples were compared with eight control samples. Multiple proteins were identified as differentially expressed (x-axis) and/or phosphorylated (y-axis). The left graph provides an overview of the expression/phosphorylation differences for all tested proteins in the two sample groups, while the right graphs show individual protein levels. The sample status is indicated as C for control or T for treated.

Reference Customers

Prof. Dr. Mathias Heikenwälder, German Cancer Research Center, Heidelberg, Germany

Dr. Caroline Pabst. University Hospital Halle, Germany

More Information

Download our scioPhospho or scioDiscover service brochure.

Information on protein and pathway content / coverage.

Get in touch to answer any remaining questions.

Ask for a quotation to get your study started.

*Minimum quantity. These values may vary depending on the sample type, study design, storage conditions. and additional parameters.