Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 1,300 proteins are analysed in parallel. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples.
| Application | Sample types - Sample requirement | Advantages | Service includes |
| Signalling Pathway Profiling | plasma/serum - 10 ul | high-content | sample preparation |
| Immunsystem activation marker profiling | tissue - 50 mg | 1030 proteins analysed | protein extraction |
| Transcription factor analysis | cells - 5 million | protein concentration measurements | |
| Cancer pathway screening | cerebrospinal fluid - 30 ul | broad coverage of: | protein quality control |
| Cell surface marker Profiling | cell culture supernatant - 10-20 ml | - signalling pathway proteins | |
| Profiling of apoptotic processes/ apoptosis pathway analysis | - transcription factors | sample labelling | |
| others on request | - apoptosis marker | sample purification | |
| Disease mechanism profiling | - markers for oxidative stress | sample incubation on arrays | |
| Pathway activation in stem cells | - cell surface markers | ||
| Analysis of organ damage/failure models | - cytokines/chemokines | raw data acquisition | |
| Response of cells and tissue models to oxidative stress analysis | data normalisation | ||
| Profiling proteomic changes for various cell culture conditions | minimal sample requirements | data analysis | |
| native matrixes (non-fractionated/non-depleted) | statistical analysis | ||
| Molecular initiate event analysis | sensitivity as ELISA or better | comprehensive study report | |
| Compound mode-of-action analysis | |||
| Prediction markers for toxicological effects | fully immuno-based assay | ||
| Biomarker Discovery | Reliable dual-colour study design | ||
| Drug Target Discovery | high reproducibility, CV < 10% | ||
| CD cell surface molecule expression in immune cells |
Target coverage - GO annotations
| Biological Process | Cellular Component | Protein Classes | |||
| cellular process (GO:0009987) | 438 | cell part (GO:0044464) | 172 | signaling molecule (PC00207) | 122 |
| metabolic process (GO:0008152) | 410 | organelle (GO:0043226) | 97 | receptor (PC00197) | 113 |
| response to stimulus (GO:0050896) | 228 | extracellular region (GO:0005576) | 60 | nucleic acid binding (PC00171) | 109 |
| biological regulation (GO:0065007) | 220 | membrane (GO:0016020) | 59 | hydrolase (PC00121) | 77 |
| developmental process (GO:0032502) | 172 | macromolecular complex (GO:0032991) | 47 | transcription factor (PC00218) | 71 |
| immune system process (GO:0002376) | 145 | extracellular matrix (GO:0031012) | 28 | enzyme modulator (PC00095) | 67 |
| localization (GO:0051179) | 116 | cell junction (GO:0030054) | 3 | cell adhesion molecule (PC00069) | 62 |
| multicellular organismal process (GO:0032501) | 72 | synapse (GO:0045202) | 2 | defense/immunity protein (PC00090) | 52 |
| apoptotic process (GO:0006915) | 67 | cytoskeletal protein (PC00085) | 40 | ||
| cellular component organization or biogenesis (GO:0071840) | 65 | oxidoreductase (PC00176) | 31 | ||
| biological adhesion (GO:0022610) | 60 | kinase (PC00137) | 31 | ||
| reproduction (GO:0000003) | 26 | transporter (PC00227) | 27 | ||
| locomotion (GO:0040011) | 4 | transfer/carrier protein (PC00219) | 25 | ||
| calcium-binding protein (PC00060) | 20 | ||||
| phosphatase (PC00181) | 20 | ||||
| structural protein (PC00211) | 18 | ||||
| extracellular matrix protein (PC00102) | 17 | ||||
| cell junction protein (PC00070) | 17 | ||||
| membrane traffic protein (PC00150) | 14 |
PLEASE NOTE: GO annotation terms may overlap
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Reference customers:
Prof. Dr. Faikah Güler, Medical School Hanover, Germany
Julian Kamhieh-Milz, Charite, Berlin, Germany
Case study - Acute Kidney Injury
