scioDiscover - Protein Expression Analysis Platform

Our scioDiscover service enables high-content, immuno-based analysis of various biological samples leading to a comprehensive overview of proteomic changes. Key pathways in various diseases such as cancer, neurological disorders and organ failure are covered. The technology was successfully applied in a variety of biomedical studies and to discover new biomarker signatures. Within the analysis, 1030 proteins are analysed in parallel. The scioDiscover protein array based service enables fast and targeted research while still offering a broad overview of potential changes on the protein level in your samples. 

 

Application Sample types - Sample requirement Advantages Service includes
       
Signalling Pathway Profiling plasma/serum - 10 ul high-content sample preparation
Immunsystem activation marker profiling tissue - 50 mg 1030 proteins analysed protein extraction
Transcription factor analysis  cells - 5 million   protein concentration measurements
Cancer pathway screening cerebrospinal fluid - 30 ul broad coverage of:  protein quality control
Cell surface marker Profiling cell culture supernatant - 10-20 ml - signalling pathway proteins  
Profiling of apoptotic processes/ apoptosis pathway analysis    - transcription factors sample labelling
  others on request - apoptosis marker sample purification
Disease mechanism profiling   - markers for oxidative stress sample incubation on arrays
Pathway activation in stem cells    - cell surface markers  
Analysis of organ damage/failure models    - cytokines/chemokines raw data acquisition
Response of cells and tissue models to oxidative stress analysis     data normalisation
Profiling proteomic changes for various cell culture conditions   minimal sample requirements data analysis
    native matrixes (non-fractionated/non-depleted) statistical analysis
Molecular initiate event analysis   sensitivity as ELISA or better comprehensive study report
Compound mode-of-action analysis      
Prediction markers for toxicological effects    fully immuno-based assay  
       
Biomarker Discovery   Reliable dual-colour study design  
Drug Target Discovery   high reproducibility, CV < 10%  
       
CD cell surface molecule expression in immune cells        

 

Target coverage - GO annotations

Biological Process   Cellular Component   Protein Classes   
cellular process (GO:0009987) 438 cell part (GO:0044464) 172 signaling molecule (PC00207) 122
metabolic process (GO:0008152) 410 organelle (GO:0043226) 97 receptor (PC00197) 113
response to stimulus (GO:0050896) 228 extracellular region (GO:0005576) 60 nucleic acid binding (PC00171) 109
biological regulation (GO:0065007) 220 membrane (GO:0016020) 59 hydrolase (PC00121) 77
developmental process (GO:0032502) 172 macromolecular complex (GO:0032991) 47 transcription factor (PC00218) 71
immune system process (GO:0002376) 145 extracellular matrix (GO:0031012) 28 enzyme modulator (PC00095) 67
localization (GO:0051179) 116 cell junction (GO:0030054) 3 cell adhesion molecule (PC00069) 62
multicellular organismal process (GO:0032501) 72 synapse (GO:0045202) 2 defense/immunity protein (PC00090) 52
apoptotic process (GO:0006915) 67     cytoskeletal protein (PC00085) 40
cellular component organization or biogenesis (GO:0071840) 65     oxidoreductase (PC00176) 31
biological adhesion (GO:0022610) 60     kinase (PC00137) 31
reproduction (GO:0000003) 26     transporter (PC00227) 27
locomotion (GO:0040011) 4     transfer/carrier protein (PC00219) 25
        calcium-binding protein (PC00060) 20
        phosphatase (PC00181) 20
        structural protein (PC00211) 18
        extracellular matrix protein (PC00102) 17
        cell junction protein (PC00070) 17
        membrane traffic protein (PC00150) 14

PLEASE NOTE: GO annotation terms may overlap 

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Reference customers:

Prof. Dr. Faikah Güler, Medical School Hanover, Germany

Julian Kamhieh-Milz, Charite, Berlin, Germany

 

Case study - Bladder Cancer

Case study - Acute Kidney Injury

 

Our service portfolio

scioPhospho: protein profiling and phosphorylation status

scioPhospho: protein profiling and phosphorylation status

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation. Features 1030 highly relevant proteins are profiled in a single assay Phosphorylation status combined with protein expression levels Tyrosine,…
Read more
scioDiscover - Protein Profiling on expression level

scioDiscover - Protein Profiling on expression level

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…
scioCD - Cell surface marker and Cytokine profiling

scioCD - Cell surface marker and Cytokine profiling

  Characterise a certain cell type?   Cell composition in a certain tissue sample?   In-depth profiling of CD-marker expression?   Increase your throughput at high sensitivity levels? You aim…
scioCyto

scioCyto

Cytokine profiling scioCyto is a high-content analysis service for cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or cell cultur supernatants…
scioUbi

scioUbi

Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse up to 1,000 proteins in a single assay.   Features Ubiquitination…

News

New article | Activated Eosinophils Exert Antitumorigenic Activities in Colorectal Cancer

| Jan 2019 | In the article, scientists at Tel Aviv University used our scioDiscover platform to analyse tumor-associated eosinophils in colorectal cancer. The full article is published in Cancer Immunology Research.

 

New article | Macrophage-derived IL-1β and TNF-α regulate arginine metabolism in neuroblastoma

| Decembre 2018 | Study by our collaborator Dr Francis Mussai at University of Birmingham suggests arginine metabolism as a target for neuroblastoma therapy. At Sciomics we are proud of having contributed to this exciting study by a scioDiscover protein profiling study. The article is published in Cancer Research.

 

New article | Protection from ischemia reperfusion injury-induced inflammation and fibrosis in C5aR2-deficient mice

| June 2018 | Complement component 5a receptor 2 (C5AR2) deficient mice are protected from inflammation and fibrosis in response to ischemia reperfusion injury. Our scioPhospho platform supported scientists at the Medical School Hannover to elucidate the underlying mechanism. The data were recently published in Kidney International.

 

New article | Modeling Congenital Adrenal Hyperplasia and Testing Interventions for Adrenal Insufficiency Using Donor-Specific Reprogrammed Cells

| January 2018 | A scioDiscover protein microarray study contributed to an article in Cell Reports. In this study Gerard Ruiz-Babot et al. analysed a donor specific reprogramming of cells.

Customer testimonial Leonardo Guasti, PhD (senior author):

This has undoubtedly opened new avenues in our research. We have found the Sciomics team to be extremely helpful and efficient during the whole process, from advising the best strategy to the final interpretation of results.

> More testimonials

A glas full of hope | Ein Glas Hoffnung

| January 2018 | Article on the StartUp Sciomics and our antibody microarray platform by our partner SCHOTT nexterion.

> English version "A glass full of hope"

> Deutsche Version "Ein Glas Hoffnung"

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