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Scientific articles

Please find below selected scientific articles on the antibody microarray platform or its application in biomedical studies for a protein profiling published in peer-reviewed journals. The articles were either published by our customers or by the founders and employees of Sciomics.

 

Selected scientific articles by our customers and partners

   

  • Awwad, M.H.S., Mahmoud, A., Bruns, H., Echchannaoui, H., Kriegsmann, K., Lutz, R., Raab, M.S., Bertsch, U., Munder, M., Jauch, A., Weisel, K., Maier, B., Weinhold, N., Salwender, H.J., Eckstein, V., Hänel, M., Fenk, R., Dürig, J., Brors, B., Benner, A., Müller-Tidow, C., Goldschmidt, H., Hundemer, M., 2021.
    Selective elimination of immunosuppressive T cells in patients with multiple myeloma.
    Leukemia 1–14. https://doi.org/10.1038/s41375-021-01172-x
  • Kowtharapu, B.S., Damaraju, J., Singh, N.K., Ziebart, J., Bader, R., Koczan, D., Stachs, O., 2021.
    Analysis of the Differential Gene and Protein Expression Profiles of Corneal Epithelial Cells Stimulated with Alternating Current Electric Fields.
    Genes 12, 299. https://doi.org/10.3390/genes12020299
  • Boberg, E., Kadri, N., Winterling, J., Davies, L.C., Björklund, A., Msghina, M., Iacobaeus, E., Le Blanc, K., 2020.
    Mental fatigue after allogeneic hematopoietic stem cell transplantation is associated with cognitive dysfunction, but not central nervous system inflammation.
    Haematologica 105, e310–e314. https://doi.org/10.3324/haematol.2019.225326
  • Fernando, R., Wardelmann, K., Deubel, S., Kehm, R., Jung, T., Mariotti, M., Vasilaki, A., Gladyshev, V.N., Kleinridders, A., Grune, T., Castro, J.P., 2020.
    Low steady-state oxidative stress inhibits adipogenesis by altering mitochondrial dynamics and decreasing cellular respiration.
    Redox Biology 32, 101507. https://doi.org/10.1016/j.redox.2020.101507
  • Fiorentino, F.P., Marchesi, I., Schröder, C., Schmidt, R., Yokota, J., Bagella, L., 2020.
    BET-Inhibitor I-BET762 and PARP-Inhibitor Talazoparib Synergy in Small Cell Lung Cancer Cells.
    Int J Mol Sci 21. https://doi.org/10.3390/ijms21249595
  • Yin, Y., Shelke, G.V., Lässer, C., Brismar, H., Lötvall, J., 2020.
    Extracellular vesicles from mast cells induce mesenchymal transition in airway epithelial cells.
    Respiratory Research 21, 101. https://doi.org/10.1186/s12931-020-01346-8
  • Fultang, L., Gamble, L.D., Gneo, L., Berry, A.M., Egan, S.A., De Bie, F., Yogev, O., Eden, G.L., Booth, S., Brownhill, S., Vardon, A., McConville, C.M., Cheng, P.N., Norris, M.D., Etchevers, H.C., Murray, J., Ziegler, D.S., Chesler, L., Schmidt, R., Burchill, S.A., Haber, M., De Santo, C., Mussai, F., 2019.
    Macrophage-Derived IL1β and TNFα Regulate Arginine Metabolism in Neuroblastoma.
    Cancer Res 79, 611–624. https://doi.org/10.1158/0008-5472.CAN-18-2139
  • Reichman, H., Itan, M., Rozenberg, P., Yarmolovski, T., Brazowski, E., Varol, C., Gluck, N., Shapira, S., Arber, N., Qimron, U., Karo-Atar, D., Lee, J.J., Munitz, A., 2019.
    Activated Eosinophils Exert Antitumorigenic Activities in Colorectal Cancer.
    Cancer Immunol Res 7, 388–400. https://doi.org/10.1158/2326-6066.CIR-18-0494
  • Kowtharapu, B.S., Murín, R., Jünemann, A.G.M., Stachs, O., 2018.
    Role of Corneal Stromal Cells on Epithelial Cell Function during Wound Healing.
    International Journal of Molecular Sciences 19, 464. https://doi.org/10.3390/ijms19020464
  • Peiro, J.L., Oria, M., Aydin, E., Joshi, R., Cabanas, N., Schmidt, R., Schroeder, C., Marotta, M., Varisco, B.M., 2018.
    Proteomic profiling of tracheal fluid in an ovine model of congenital diaphragmatic hernia and fetal tracheal occlusion.
    Am J Physiol Lung Cell Mol Physiol 315, L1028–L1041. https://doi.org/10.1152/ajplung.00148.2018
  • Thorenz, A., Derlin, K., Schröder, C., Dressler, L., Vijayan, V., Pradhan, P., Immenschuh, S., Jörns, A., Echtermeyer, F., Herzog, C., Chen, R., Rong, S., Bräsen, J.H., Kooten, C. van, Kirsch, T., Klemann, C., Meier, M., Klos, A., Haller, H., Hensen, B., Gueler, F., 2018.
    Enhanced activation of interleukin-10, heme oxygenase-1, and AKT in C5aR2-deficient mice is associated with protection from ischemia reperfusion injury–induced inflammation and fibrosis.
    Kidney International 94, 741–755. https://doi.org/10.1016/j.kint.2018.04.005
  • Hamidi, Z., Tejero, E., Schmidt, R., Tucker, R., Pedro, A., 2017.
    Identification of potential blood-derived extracellular vesicles biomarkers to diagnose and predict distant metastases in ER+ breast cancer patients.
    bioRxiv 202291. https://doi.org/10.1101/202291
  • Sass, F.A., Schmidt‐Bleek, K., Ellinghaus, A., Filter, S., Rose, A., Preininger, B., Reinke, S., Geissler, S., Volk, H.-D., Duda, G.N., Dienelt, A., 2017.
    CD31+ Cells From Peripheral Blood Facilitate Bone Regeneration in Biologically Impaired Conditions Through Combined Effects on Immunomodulation and Angiogenesis.
    Journal of Bone and Mineral Research 32, 902–912. https://doi.org/10.1002/jbmr.3062
  • Bakdash, G., Buschow, S.I., Gorris, M.A.J., Halilovic, A., Hato, S.V., Sköld, A.E., Schreibelt, G., Sittig, S.P., Torensma, R., Duiveman-de Boer, T., Schröder, C., Smits, E.L., Figdor, C.G., de Vries, I.J.M., 2016.
    Expansion of a BDCA1+CD14+ Myeloid Cell Population in Melanoma Patients May Attenuate the Efficacy of Dendritic Cell Vaccines.
    Cancer Res 76, 4332–4346. https://doi.org/10.1158/0008-5472.CAN-15-1695

 

Selected scientific articles by our founders and employees

  • Fiorentino, F.P., Marchesi, I., Schröder, C., Schmidt, R., Yokota, J., Bagella, L., 2020.
    BET-Inhibitor I-BET762 and PARP-Inhibitor Talazoparib Synergy in Small Cell Lung Cancer Cells.
    International Journal of Molecular Sciences 21, 9595. https://doi.org/10.3390/ijms21249595
  • Fultang, L., Gamble, L.D., Gneo, L., Berry, A.M., Egan, S.A., Bie, F.D., Yogev, O., Eden, G.L., Booth, S., Brownhill, S., Vardon, A., McConville, C.M., Cheng, P.N., Norris, M.D., Etchevers, H.C., Murray, J., Ziegler, D.S., Chesler, L., Schmidt, R., Burchill, S.A., Haber, M., Santo, C.D., Mussai, F., 2019.
    Macrophage-Derived IL1β and TNFα Regulate Arginine Metabolism in Neuroblastoma.
    Cancer Res 79, 611–624. https://doi.org/10.1158/0008-5472.CAN-18-2139
  • Thorenz, A., Derlin, K., Schröder, C., Dressler, L., Vijayan, V., Pradhan, P., Immenschuh, S., Jörns, A., Echtermeyer, F., Herzog, C., Chen, R., Rong, S., Bräsen, J.H., Kooten, C. van, Kirsch, T., Klemann, C., Meier, M., Klos, A., Haller, H., Hensen, B., Gueler, F., 2018.
    Enhanced activation of interleukin-10, heme oxygenase-1, and AKT in C5aR2-deficient mice is associated with protection from ischemia reperfusion injury–induced inflammation and fibrosis.
    Kidney International 94, 741–755. https://doi.org/10.1016/j.kint.2018.04.005
  • Peiro, J.L., Oria, M., Aydin, E., Joshi, R., Cabanas, N., Schmidt, R., Schroeder, C., Marotta, M., Varisco, B.M., 2018.
    Proteomic profiling of tracheal fluid in an ovine model of congenital diaphragmatic hernia and fetal tracheal occlusion.
    American Journal of Physiology-Lung Cellular and Molecular Physiology 315, L1028–L1041. https://doi.org/10.1152/ajplung.00148.2018
  • Bakdash, G., Buschow, S.I., Gorris, M.A.J., Halilovic, A., Hato, S.V., Sköld, A.E., Schreibelt, G., Sittig, S.P., Torensma, R., Boer, T.D., Schröder, C., Smits, E.L., Figdor, C.G., Vries, I.J.M. de, 2016.
    Expansion of a BDCA1+CD14+ Myeloid Cell Population in Melanoma Patients May Attenuate the Efficacy of Dendritic Cell Vaccines.
    Cancer Res 76, 4332–4346. https://doi.org/10.1158/0008-5472.CAN-15-1695
  • Kibat, J., Schirrmann, T., Knape, M.J., Helmsing, S., Meier, D., Hust, M., Schröder, C., Bertinetti, D., Winter, G., Pardes, K., Funk, M., Vala, A., Giese, N., Herberg, F.W., Dübel, S., Hoheisel, J.D., 2016.
    Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality.
    N Biotechnol 33, 574–581. https://doi.org/10.1016/j.nbt.2015.12.003
  • Betzen, C., Alhamdani, M.S.S., Lueong, S., Schröder, C., Stang, A., Hoheisel, J.D., 2015.
    Clinical proteomics: Promises, challenges and limitations of affinity arrays.
    PROTEOMICS – Clinical Applications 9, 342–347. https://doi.org/10.1002/prca.201400156
  • Nijaguna, M.B., Schröder, C., Patil, V., Shwetha, S.D., Hegde, A.S., Chandramouli, B.A., Arivazhagan, A., Santosh, V., Hoheisel, J.D., Somasundaram, K., 2015.
    Definition of a serum marker panel for glioblastoma discrimination and identification of Interleukin 1β in the microglial secretome as a novel mediator of endothelial cell survival induced by C-reactive protein.
    Journal of Proteomics 128, 251–261. https://doi.org/10.1016/j.jprot.2015.07.026
  • Srinivasan, H., Allory, Y., Sill, M., Vordos, D., Alhamdani, M.S.S., Radvanyi, F., Hoheisel, J.D., Schröder, C., 2014.
    Prediction of recurrence of non muscle-invasive bladder cancer by means of a protein signature identified by antibody microarray analyses.
    PROTEOMICS 14, 1333–1342. https://doi.org/10.1002/pmic.201300320
  • Hoheisel, J.D., Alhamdani, M.S.S., Schröder, C., 2013.
    Affinity-based microarrays for proteomic analysis of cancer tissues.
    PROTEOMICS – Clinical Applications 7, 8–15. https://doi.org/10.1002/prca.201200114
  • Schröder, C., Srinivasan, H., Sill, M., Linseisen, J., Fellenberg, K., Becker, N., Nieters, A., Hoheisel, J.D., 2013.
    Plasma protein analysis of patients with different B-cell lymphomas using high-content antibody microarrays.
    PROTEOMICS – Clinical Applications 7, 802–812. https://doi.org/10.1002/prca.201300048
  • Alhamdani, M.S.S., Youns, M., Buchholz, M., Gress, T.M., Beckers, M.-C., Maréchal, D., Bauer, A., Schröder, C., Hoheisel, J.D., 2012.
    Immunoassay-based proteome profiling of 24 pancreatic cancer cell lines.
    Journal of Proteomics, 3747–3759. https://doi.org/10.1016/j.jprot.2012.04.042
  • Alhamdani, M.S.S., Schröder, C., Hoheisel, J.D., 2010.
    Analysis conditions for proteomic profiling of mammalian tissue and cell extracts with antibody microarrays.
    PROTEOMICS 10, 3203–3207. https://doi.org/10.1002/pmic.201000170
  • Alhamdani, M.S.S., Schröder, C., Werner, J., Giese, N., Bauer, A., Hoheisel, J.D., 2010.
    Single-Step Procedure for the Isolation of Proteins at Near-Native Conditions from Mammalian Tissue for Proteomic Analysis on Antibody Microarrays.
    J. Proteome Res. 9, 963–971. https://doi.org/10.1021/pr900844q
  • Schröder, C., Jacob, A., Tonack, S., Radon, T.P., Sill, M., Zucknick, M., Rüffer, S., Costello, E., Neoptolemos, J.P., Crnogorac-Jurcevic, T., Bauer, A., Fellenberg, K., Hoheisel, J.D., 2010.
    Dual-color Proteomic Profiling of Complex Samples with a Microarray of 810 Cancer-related Antibodies.
    Molecular & Cellular Proteomics 9, 1271–1280. https://doi.org/10.1074/mcp.M900419-MCP200
  • Sill, M., Schröder, C., Hoheisel, J.D., Benner, A., Zucknick, M., 2010.
    Assessment and optimisation of normalisation methods for dual-colour antibody microarrays.
    BMC Bioinformatics 11, 556. https://doi.org/10.1186/1471-2105-11-556
  • Kusnezow, W., Banzon, V., Schröder, C., Schaal, R., Hoheisel, J.D., Rüffer, S., Luft, P., Duschl, A., Syagailo, Y.V., 2007.
    Antibody microarray-based profiling of complex specimens: systematic evaluation of labeling strategies.
    PROTEOMICS 7, 1786–1799. https://doi.org/10.1002/pmic.200600762

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scioPhospho: protein profiling and phosphorylation status

scioPhospho: protein profiling and phosphorylation status

scioPhospho combines the advantages of a robust and cost-efficient protein expression profiling using scioDiscover with information on phosphorylation status. This combination provides a comprehensive overview on signalling events and pathway activity regulation. Features 1,300 highly relevant proteins are profiled in a single assay Phosphorylation status combined with protein expression levels…
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scioDiscover - Protein Profiling on expression level

scioDiscover - Protein Profiling on expression level

Knowledge about protein expression levels is of utmost importance to predict toxic effects, estimate adverse effects of drug candidates and to identify new drug targets. Sciomics has a highly optimised…
scioCD - Cell surface marker and Cytokine profiling

scioCD - Cell surface marker and Cytokine profiling

  Characterise a certain cell type?   Cell composition in a certain tissue sample?   In-depth profiling of CD-marker expression?   Increase your throughput at high sensitivity levels? You aim…
scioUbi

scioUbi

Protein & ubiquitination profiling scioUbi is a high-content protein expression and ubiquitination level analysis. With scioUbi you can analyse up to 1,000 proteins in a single assay.   Features Screen…
scioCyto : Cytokine profiling

scioCyto : Cytokine profiling

scioCyto : Cytokine profiling scioCyto is a high-content analysis service for multiplex cytokine and chemokine profiling on protein level. A great variety of samples such as plasma, tissue, cells or…

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News

New article | Selective elimination of immunosuppressive T cells in patients with multiple myeloma

| Feb 2021 | Scientists at Heidelberg University Hospital used our scioCD  platform to profile CD8+SLAMF7+ cell cultures:

"High throughput screening of 351 different cytokines and immune proteins in the supernatants of the T cells cultures revealed strong upregulation of IL-6, IL-8, both vital survival factors for myeloma cells, and CXCL5, a chemokine that could enhance the frequency of CD4 Treg, in the cultures containing CD8+SLAMF7+ cells. IL-2 and IL-5 were upregulated in the control cultures without CD8+SLAMF7+ T cells, highlighting a more activated state in the control group and suggestion IL-6 and IL-8 as potential effector cytokines for the suppressive CD8+SLAMF7+ T cells."

Awwad, M. H. S. et al. (2021) ‘Selective elimination of immunosuppressive T cells in patients with multiple myeloma’, Leukemia, pp. 1–14. doi: 10.1038/s41375-021-01172-x.

 

New article | Analysis of the Differential Gene and Protein Expression Profiles of Corneal Epithelial Cells Stimulated with Alternating Current Electric Fields

| Feb 2021 | Scientists at Rostock University Medical Center used our scioCD  platform to Corneal Epithelial Cells stimulated with Alternating Current Electric Fields:

"Analysis of the protein array data revealed that the treatment of cells with AC EFs altered the expression of various protein products ... revealed activation of various diverse cellular signaling pathways in which TNF, MAPK, IL17, and PI3K-Akt signaling pathways were significantly impacted ..."

B. S. Kowtharapu et. al., „Analysis of the Differential Gene and Protein Expression Profiles of Corneal Epithelial Cells Stimulated with Alternating Current Electric Fields“, Genes, Bd. 12, Nr. 2, Art. Nr. 2, Feb. 2021, doi: 10.3390/genes12020299.

 

New article | BET-Inhibitor I-BET762 and PARP-Inhibitor Talazoparib Synergy in Small Cell Lung Cancer Cells

| Dec 2020 | Scientists at University of Sassari used our scioPhospho  platform to to analyse the synergy of BET and PARP inhibition in Small Cell Lung Cancer Cells:

"Among the I-BET762-associated and combination-associated differentially expressed proteins, 150 were common to both treatment conditions. ... Ranking I-BET762-associated and combination-associated DEPs by their differential expression revealed CHEK2 and PTEN to be among the top-downregulated proteins, whose deficiencies have been associated with HR defects and increased PARPi sensitivity. "

Fiorentino, F. P. et al. (2020) ‘BET-Inhibitor I-BET762 and PARP-Inhibitor Talazoparib Synergy in Small Cell Lung Cancer Cells’, International Journal of Molecular Sciences, 21(24). doi: 10.3390/ijms21249595.

 

New article | Activated Eosinophils Exert Antitumorigenic Activities in Colorectal Cancer

| Jan 2019 | In the article, scientists at Tel Aviv University used our scioDiscover platform to analyse tumor-associated eosinophils in colorectal cancer. The full article is published in Cancer Immunology Research.

 

New article | Protection from ischemia reperfusion injury-induced inflammation and fibrosis in C5aR2-deficient mice

| June 2018 | Complement component 5a receptor 2 (C5AR2) deficient mice are protected from inflammation and fibrosis in response to ischemia reperfusion injury. Our scioPhospho platform supported scientists at the Medical School Hannover to elucidate the underlying mechanism. The data were recently published in Kidney International.

 

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Prof. Claudio Mauro
Birmingham University, Institute of Inflammation and Ageing, Birmingham, UK

"We have performed an acetylation array on primary T lymphocytes with Sciomics. The quality of the data we have obtained is exceptional. They have a model of collaboration with the team conducting the experiment. This means they generate the data and assist with further analyses of the data. The screening has opened up several exciting ways forward for us."

Product: scioAcetyl protein and protein acetylation profiling service (under co-development with selected partners)

 

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